ku70/80 ku7000 ku70 antibody ku70 uniprot ku7000 specs ku70/80 function ku70ut7000fxkr ku70ut7080fxkr ku70 and ku80 function Please note that this site uses cookies to personalise content and adverts, to provide social media features, and to analyse web traffic.

InteraktionerRedigera. Werner syndrom ATP-beroende helikas har visat sig interagera med: BLM; DNA-PKcs ,; FEN1 ,; Ku70 ,; Ku80 ,; P53 ,; PCNA ,; TERF2 ,

Uniprot Id: P12956: NCBI Gene Id: 2547: Species Of This Entry: Human: Gene Name: XRCC6: Protein Name: X-ray repair cross-complementing protein 6: Superfamily: ku70 family Invitrogen Anti-Ku70 Polyclonal, Catalog # PA5-25915. Tested in Western Blot (WB), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human, Mouse samples. Supplied as 400 µL purified antibody (2 mg/mL). Western blot was performed using Anti-Ku70 Recombinant Rabbit Monoclonal Antibody (JM61-31)(Product # MA5-32645) and a 70kDa band corresponding to Ku70 was observed across cell lines tested. Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), Jurkat (Lane 3), HEK-293 (Lane 4), Hep G2 (Lane 5) and U-2 OS (Lane 6) were The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8).

Ku70 uniprot

Knockdown of Ku70 was achieved by transfecting HeLa cells with Ku70 specific siRNAs (Silencer® select Product # s5457, s5455). Western blot analysis (Fig. a) was performed using nuclear enriched cell extracts from the Ku70 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). HSF1 interacted directly with both of the N-terminal sequences of the Ku70 and Ku86 proteins, which inhibited the endogenous heterodimeric interaction between Ku70 and Ku86. The blocking of the Ku70 and Ku86 interaction by HSF1 induced defective NHEJ repair activity and ultimately activated genomic instability after ionizing radiation (IR), which was similar to effects seen in Ku70 or Ku80 Invitrogen Anti-Phospho-Ku70 (Ser5) Polyclonal, Catalog # PA5-40247. Tested in Western Blot (WB) applications. This antibody reacts with Human, Rat samples.

X-ray repair cross complementing 6 [Source:HGNC Symbol;Acc: HGNC:4055]. Gene Synonyms.

Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. Additional components of the NHEJ complex include NHEJ1/XLF and PAXX.

Lupus Ku autoantigen protein p70 (Ku70) is also known as X- Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry Mar 1, 2021 X-ray repair cross-complementing protein 6, 5'-dRP/AP lyase Ku70, 5'- deoxyribose-5-phosphate lyase Ku70, ATP-dependent DNA helicase 2 Polyclonal Antibody for studying Ku70 in the Cell Cycle / Checkpoint research area.

Ku70 is the 70 kDa subunit of the lupus Ku autoantigen. The lupus Ku autoantigen was identified in individuals with systemic lupus erythematosus (SLE) and related disorders. The Ku antigen is a heterodimer of Ku70 and Ku80. The Ku70/Ku80 complex functions as a single-stranded DNA-dependent ATP-dependent helicase.

ku70, of the ku70/ku80 heterodimer, binds to the stem loop of tlc1, the RNA component of telomerase. Required for mating-type switching (By similarity). KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance.

Uniprot: P12956 NCBI: NM_001469 NP PA5-27538 immunoprecipitates Ku70 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of Ku70 protein by 2.5 µg of Ku70 antibody (Product # PA5-27538) on 7.5% SDS-PAGE.
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This antibody reacts with Human, Mouse samples.

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KU70 - ATP-dependent DNA helicase 2 subunit KU70 - Arabidopsis thaliana (Mouse-ear cress) - KU70 gene & protein UniProtKB - Q9FQ08 (KU70_ARATH)

We used the amino acid sequences of Ku70 orthologs from S. cerevisiae (UniProt ID PLA0263 Sigma-Aldrich Rabbit anti-Ku70 Antibody, Affinity Purified Powered by Bethyl Laboratories, Inc. Synonym: 5′-dRP lyase Ku70, 5′-deoxyribose-5-phosphate lyase Ku70, 70 kDa subunit of Ku antigen, 70 kDa subunit, 70kDa, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase II, CTC box binding factor 75 kDa subunit, CTC box KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. UniProt is an ELIXIR core data resource. 2001-03-01 · Single-stranded DNA-dependent ATP-dependent helicase. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance.

Two of these proteins, Ku70 and Ku80, were identified by immunoblot analysis. The third polypeptide, which was identified by mass spectrometry analysis, is identical to poly(ADP-ribose) polymerase-1(PARP-1), a 113-kDa enzyme that functions as a sensor of DNA damage.

Uniprot: >>Visit The Human Protein Atlas. Gene ID: Gene Name: XRCC6.

Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU70, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA (By similarity). Ku70 and Ku80 have also been experimentally characterized in plants, where they appear to play a similar role to that in other eukaryotes. In rice, suppression of either protein has been shown to promote homologous recombination (HR) [13] This effect was exploited to improve gene targeting (GT) efficiency in Arabidopsis thaliana . Ku70 stimulates fusion of dysfunctional telomeres yet protects chromosome ends from homologous recombination. Celli G.B., Denchi E.L., de Lange T. Ku70-Ku80 heterodimers promote the non-homologous end-joining (NHEJ) of DNA breaks and, as shown here, the fusion of dysfunctional telomeres.